The ultimate aim is the use of this in vivo injury model to test novel ocular bandages and substrate treatments. Methods De-epithelialisation first required in vitro optimisation for reproducibility before use in in vivo rabbit models. Corneas obtained from New Zealand White rabbits were de-epithelialised with sodium hydroxide (NaOH) solution using a range of concentrations, durations and modes of application. selleck chemical
The injury area, depth and severity were assessed using haematoxylin and eosin staining and transmission electron microscopy. The optimised in vitro model was applied to an in vivo pilot study in rabbits and tested for reproducibility using normal and fluorescein slit lamp images. Results In vitro optimisation revealed that higher NaOH concentrations and extended durations caused undesirable irreversible damage to the cornea. Lower concentrations and durations produced U0126
the injury required. The injury model was established in an in vivo pilot study using our optimal parameters and tested to see if a chemical burn with little variation in area or severity could be achieved. Following 48 hour treatment we were able to detect reliable wound healing using our refined model. Conclusion The in vitro optimisation showed us parameters that were sufficient to de-epithelialise the cornea, creating an injury without causing stromal damage. The model is now in use in vivo, to test the biocompatibility and efficacy of various ocular surface substrate treatments. ""Purpose Purpose: Under night driving conditions visual performance is compromised and become worse with disability glare, age and certain ocular pathologies. The aim of this study was to analyze mesopic visual function of young subjects. Methods Methods: The sample included 37 right eyes of healthy young adults (22.2 �� 1.8 years). The selected criteria were: Best-corrected VA of at least 0.04 logMAR, and refraction �ܡ� 3.75. Under mesopic (0.1 to 0.2 cd/m2) luminance conditions and with the best-optical correction in the right eye, the logMAR VA was measured using high-contrast (HC-96%) and low-contrast (LC-10%) logMAR Bailey-Lovie letter charts, and the mesopic contrast sensitivity (CS) without / with glare Vemurafenib cell line
was assessed with Mesotest II. The intraocular straylight was measured with the C-Quant. Results Results: Under mesopic conditions, the HC and LC logMAR VA was 0.28 �� 0.1 and 0.70 �� 0.1 respectively, showing a significant correlation between them (Pearson��s correlation = 0.75; p< 0.00); The mesopic log CS without glare was 0.19 �� 0.1 and with glare was 0.11 �� 0.1. Disability glare induced a 42% decrease in mesopic log CS (p < 0.00). LC logMAR VA was significant correlated with mesopic log CS without and with glare (Pearson��s correlation = 0.4, p< 0.02, and 0.42, p< 0.02 respectively). The intraocular straylight Log (s) was 0.87 �� 0.1, but did not show any significant association with logMAR VA nor the log CS without and with glare under mesopic luminance conditions.